Central project 1 Eye Imaging

Cologne Experimental Eye Imaging Center (CEE-IC)


The central aim of the proposed research consortium is the identification and analysis of inflammation-associated mechanisms of ocular diseases. As all projects investigate cellular processes of inflammation in particular non- or minimally-invasive intravital imaging techniques are necessary, which in real time can capture and follow up the induced alterations both in in vitro and in vivo experiments. The CEE-IC will provide an experimental imaging facility equipped with different OCT and fluorescence angiography devices together with stereomicroscopy that will enable state-of-the-art examination of the target structures without tampering the results by tissue probing or phototoxic effects. Furthermore the C1-project will provide well-established advanced vessel and particle analysis methods and will develop further standardized, highly qualitative data analysis methods in close cooperation with the individual projects. Thereby highly reliable and innovative data sets will be generated.


Cursiefen C, Bock F, Clahsen T, Regenfuss B, Reis A, Steven P, Heindl LM, Bosch JJ, Hos D, Eming S, Grajewski R, Heiligenhaus A, Fauser S, Austin J, Langmann T. [New Therapeutic Approaches in Inflammatory Diseases of the Eye – Targeting Lymphangiogenesis and Cellular Immunity: Research Unit FOR 2240 Presents Itself]. Klin Monbl Augenheilkd. 2017 May;234(5):679-685.

Steven P, Braun T, Krösser S, Gehlsen U. [Influence of Aging on Severity and Anti-Inflammatory Treatment of Experimental Dry Eye Disease]. Klin Monbl Augenheilkd. 2017 May;234(5):662-669.

Gehlsen U, Braun T, Notara M, Krösser S, Steven P. A semifluorinated alkane (F4H5) as novel carrier for cyclosporine A: a promising therapeutic and prophylactic option for topical treatment of dry eye. Graefes Arch Clin Exp Ophthalmol. 2017 Apr;255(4):767-775.

Horstmann J, Siebelmann S, Schulz-Hildebrandt H, Glasunow I, Schadschneider A, Hüttmann G. [Understanding OCT – Part 2: State of the Practice]. Klin Monbl Augenheilkd. 2017 Feb;234(2):233-247.

Horstmann J, Siebelmann S, Schulz-Hildebrandt H, Glasunow I, Schadschneider A, Hüttmann G. [Understanding OCT – Part 1: Basic Knowledge]. Klin Monbl Augenheilkd. 2017 Jan;234(1):131-143.

Siebelmann S, Gehlsen U, Le Blanc C, Stanzel TP, Cursiefen C, Steven P. Detection of graft detachments immediately following Descemet membrane endothelial keratoplasty (DMEK) comparing time domain and spectral domain OCT. Graefes Arch Clin Exp Ophthalmol. 2016 Dec;254(12):2431-2437.

Siebelmann S, Bachmann B, Lappas A, Dietlein T, Hermann M, Roters S, Cursiefen C, Steven P. [Intraoperative optical coherence tomography in corneal and glaucoma surgical procedures]. Ophthalmologe. 2016 Aug;113(8):646-50.

Siebelmann S, Bachmann B, Lappas A, Dietlein T, Steven P, Cursiefen C.[Intraoperative optical coherence tomography for examination of newborns and infants under general anesthesia]. Ophthalmologe. 2016 Aug;113(8):651-5.

Siebelmann S, Steven P, Cursiefen C. [Intraoperative Optical Coherence Tomography In Deep Anterior Lamellar Keratoplasty]. Klin Monbl Augenheilkd. 2016 Jun;233(6):717-21.

Tahmaz V, Gehlsen U, Sauerbier L, Holtick U, Engel L, Radojska S, Petrescu-Jipa VM, Scheid C, Hallek M, Gathof B, Cursiefen C, Steven P. Treatment of severe chronic ocular graft-versus-host disease using 100% autologous serum eye drops from a sealed manufacturing system: a retrospective cohort study. Br J Ophthalmol. 2016 Jun 6. pii: bjophthalmol-2015-307666.

Siebelmann S, Steven P, Cursiefen C (2015) Intraoperative Optical Coherence Tomography: Ocular Surgery on a Higher Level or Just Nice Pictures? JAMA Ophthalmol.

Engel LA, Wittig S, Bock F, Sauerbier L, Scheid C, Holtick U, Chemnitz JM, Hallek M, Cursiefen C, Steven P (2015) Meibography and meibomian gland measurements in ocular graft-versus-host disease. Bone Marrow Transplant.50(7):961-7.

Gehlsen U, Szaszák M, Gebert A, Koop N, Hüttmann G, Steven P (2015) Non-Invasive Multi-Dimensional Two-Photon Microscopy enables optical fingerprinting (TPOF) of immune cells. J Biophotonics. 8(6):466-79.

Siebelmann S, Gehlsen U, Hüttmann G, Koop N, Bölke T, Gebert A, Stern ME, Niederkorn JY, Steven P (2013) Development, alteration and real time dynamics of conjunctiva-associated lymphoid tissue. PLoS One. 8:e82355.

Steven P, Le Blanc C, Velten K, Lankenau E, Krug M, Oelckers S, Heindl LM, Gehlsen U, Hüttmann G, Cursiefen C (2013) Optimizing descemet membrane endothelial keratoplasty using intraoperative optical coherence tomography. JAMA Ophthalmol. 131:1135-42.

Gehlsen U, Oetke A, Szaszák M, Koop N, Paulsen F, Gebert A, Huettmann G, Steven P (2012) Two-photon fluorescence lifetime imaging monitors metabolic changes during wound healing of corneal epithelial cells in vitro. Graefes Arch Clin Exp Ophthalmol. 250:1293-302.

Steven P, Bock F, Hüttmann G, Cursiefen C (2011) Intravital two-photon microscopy of immune cell dynamics in corneal lymphatic vessels. PLoS One. 6:e26253.

Steven P, Rupp J, Hüttmann G, Koop N, Lensing C, Laqua H, Gebert A (2008) Experimental induction and three-dimensional two-photon imaging of conjunctiva-associated lymphoid tissue. Invest Ophthalmol Vis Sci. 49:1512-7.