Project 1 Lymph-Transplant

Induction of corneal transplant tolerance by anti(lymph)angiogenic therapy

 
 
 
 

Summary

Corneal grafting (penetrating keratoplasty) is the most frequently performed transplantation worldwide. Immune reactions are the main complication after grafting into pathologically prevascularized, so called high-risk recipient eyes. We previously showed that clinically invisible lymphatic vessels play a key role in inducing corneal graft rejection and that anti(lymph)angiogenic therapy promotes graft survival in the murine model of transplantation. But the precise immunological mechanisms whereby antilymphangiogenesis promotes graft survival are only poorly understood. Our hypothesis is that the blockade of lymphangiogenesis leads to graft tolerance (rather than ignorance) due to prolonged retention and immunomodulation of antigen presenting cells in the recipient bed. Therefore, one key aim of this project is to analyse whether and when corneal antilymphangiogenic therapy leads to graft tolerance by an altered maturation status of antigen-presenting cells and/or to the induction of tolerogenic effector cells (regulatory T cells) in the absence of lymphatic vessels. Currently available anti(lymph)angiogenic therapies have the drawback that they only act on actively proliferating vessels. But most patients undergoing high-risk transplantation already have established mature pathologic corneal blood and lymphatic vessels. So far no lymphangioregressive strategies are available and the mechanisms governing lymphatic vessel maturation are not understood. Our second aim therefore is to develop strategies to regress preexisting pathologic corneal lymphatic vessels by destruction (using photodynamic therapy or diathermy) or by molecular destabilisation of the lymphatic endothelial cells. Overall this project will lead to a better understanding of the precise mechanisms whereby antilymphangiogenesis promotes transplant survival and to the development of novel strategies to regress mature lymphatic vessels in the high-risk scenario to subsequently promote graft survival.

 
 
 

Selected Key Publications of Project 1

 
 
 

More Publications of Project 1

Schaub F, Bachmann BO, Seyeddain O, Moussa S, Reitsamer HA, Cursiefen C. [Mid- and Longterm Experiences with the Boston-Keratoprosthesis. The Cologne and Salzburg Perspective]. Klin Monbl Augenheilkd. 2017 Jun;234(6):770-775.

Matthaei M, Sandhaeger H, Hermel M, Adler W, Jun AS, Cursiefen C, Heindl LM. Changing Indications in Penetrating Keratoplasty: A Systematic Review of 34 Years of Global Reporting. Transplantation. 2017 Jun;101(6):1387-1399.

Schrittenlocher S, Penier M, Schaub F, Bock F, Cursiefen C, Bachmann B. Intraocular Lens Calcifications after (Triple-) Descemet Membrane Endothelial Keratoplasty (DMEK). Am J Ophthalmol. 2017 May 5. pii: S0002-9394(17)30193-9.

Cursiefen C, Bock F, Clahsen T, Regenfuss B, Reis A, Steven P, Heindl LM, Bosch JJ, Hos D, Eming S, Grajewski R, Heiligenhaus A, Fauser S, Austin J, Langmann T. [New Therapeutic Approaches in Inflammatory Diseases of the Eye – Targeting Lymphangiogenesis and Cellular Immunity: Research Unit FOR 2240 Presents Itself]. Klin Monbl Augenheilkd. 2017 May;234(5):679-685.

Cursiefen C, Heiligenhaus A. [“Translational Research in Ophthalmology is Important!”]. Klin Monbl Augenheilkd. 2017 May;234(5):650-651.

Enders P, Holtick U, Schaub F, Tuchscherer A, Hermann MM, Scheid C, Cursiefen C, Bachmann BO. Corneal Densitometry for Quantification of Corneal Deposits in Monoclonal Gammopathies. Cornea. 2017 Apr;36(4):470-475.

Schaub F, Enders P, Bachmann BO, Heindl LM, Cursiefen C. Effect of corneal collagen crosslinking on subsequent deep anterior lamellar keratoplasty (DALK) in keratoconus. Graefes Arch Clin Exp Ophthalmol. 2017 Apr;255(4):811-816.

Hos D, Tuac O, Schaub F, Stanzel TP, Schrittenlocher S, Hellmich M, Bachmann BO, Cursiefen C. Incidence and Clinical Course of Immune Reactions after Descemet Membrane Endothelial Keratoplasty: Retrospective Analysis of 1000 Consecutive Eyes. Ophthalmology. 2017 Apr;124(4):512-518.

Lorenz K, Scheller Y, Bell K, Grus F, Ponto KA, Bock F, Cursiefen C, Flach J, Gehring M, Peto T, Silva R, Tal Y, Pfeiffer N. A prospective, randomised, placebo-controlled, double-masked, three-armed, multicentre phase II/III trial for the Study of a Topical Treatment of Ischaemic Central Retinal Vein Occlusion  to Prevent Neovascular Glaucoma – the STRONG study: study protocol for a randomised controlled trial. Trials. 2017 Mar 16;18(1):128.

Schaub F, Enders P, Roters S, Cursiefen C, Bachmann BO. Single-pass Ultrathin DSAEK (UT-DSAEK) with the SLc Expert Microkeratome(®). Acta Ophthalmol. 2017 Mar;95(2):e160-e161.

Schaub F, Enders P, Bluhm C, Bachmann BO, Cursiefen C, Heindl LM. Two-Year Course of Corneal Densitometry After Descemet Membrane Endothelial Keratoplasty. Am J Ophthalmol. 2017 Mar;175:60-67.

Schaub F, Simons HG, Enders P, Bachmann BO, Roters S, Cursiefen C, Heindl LM. [Corneal donation : Dilemma between growing demand and declining donor rate]. Ophthalmologe. 2016 Dec;113(12):1058-1065.

Schaub F, Heindl LM, Enders P, Roters S, Bachmann BO, Cursiefen C. [Deep Anterior Lamellar Keratoplasty : Experiences and results of the first 100 consecutive DALK from the University Eye Hospital of Cologne]. Ophthalmologe. 2016 Dec 21. doi: 10.1007/s00347-016-0424-4. [Epub ahead of print]

Hoerster R, Stanzel TP, Bachmann BO, Siebelmann S, Cursiefen C. Intensified Early Postoperative Topical Steroids Do Not Influence Endothelial Cell Density After Descemet Membrane Endothelial Keratoplasty Combined With Cataract Surgery (Triple-DMEK). Cornea. 2016 Nov;35(11):1396-1400.

Hos D, Dörrie J, Schaft N, Bock F, Notara M, Kruse FE, Krautwald S, Cursiefen C, Bachmann BO. Blockade of CCR7 leads to decreased dendritic cell migration to draining lymph nodes and promotes graft survival in low-risk corneal transplantation. Exp Eye Res. 2016 May;146:1-6.

Hoerster R, Stanzel TP, Bachmann BO, Siebelmann S, Felsch M, Cursiefen C. Intensified Topical Steroids as Prophylaxis for Macular Edema After Posterior Lamellar Keratoplasty Combined With Cataract Surgery. Am J Ophthalmol. 2016 Mar;163:174-9.

Hos D, Bucher F, Regenfuss B, Dreisow ML, Bock F, Heindl LM, Eming SA, Cursiefen C. IL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via Macrophages. Am J Pathol. 2016 Jan;186(1):159-71.

Siebelmann S, Hermann M, Dietlein T, Bachmann B, Steven P, Cursiefen C. Intraoperative Optical Coherence Tomography in Children with Anterior Segment Anomalies. Ophthalmology. 2015 Dec;122(12):2582-4.

Notara M, Refaian N, Braun G, Steven P, Bock F, Cursiefen C. Short-term uvb-irradiation leads to putative limbal stem cell damage and niche cell-mediated upregulation of macrophage recruiting cytokines. Stem Cell Res. 2015 Nov;15(3):643-54.

Heindl LM, Kaser-Eichberger A, Schlereth SL, Bock F, Regenfuss B, Reitsamer HA, McMenamin P, Lutty GA, Maruyama K, Chen L, Dana R, Kerjaschki D, Alitalo K, De Stefano ME, Junghans BM, Schroedl F, Cursiefen C. Sufficient Evidence for Lymphatics in the Developing and Adult Human Choroid? Invest Ophthalmol Vis Sci. 2015 Oct;56(11):6709-10.

Schöllhorn L, Bock F, Cursiefen C. Thrombospondin-1 as a Regulator of Corneal Inflammation and Lymphangiogenesis: Effects on Dry Eye Disease and Corneal Graft Immunology. J Ocul Pharmacol Ther. 2015 Sep;31(7):376-85.

Schaub F, Cursiefen C, Heindl LM. Retrospective Appraisal of Split-Cornea Transplantation: An Audit of 1141 Donor Corneas. JAMA Ophthalmol. 2015 Sep;133(9):1086-7.

Bucher F, Hos D, Müller-Schwefe S, Steven P, Cursiefen C, Heindl LM. Spontaneous long-term course of persistent peripheral graft detachments after Descemet’s membrane endothelial keratoplasty. Br J Ophthalmol. 2015 Jun;99(6):768-72.

Lee HS, Hos D, Blanco T, Bock F, Reyes NJ, Mathew R, Cursiefen C, Dana R, Saban DR. Involvement of corneal lymphangiogenesis in a mouse model of allergic eye disease. Invest Ophthalmol Vis Sci. 2015 May;56(5):3140-8.

Hos D, Schlereth SL, Bock F, Heindl LM, Cursiefen C. Antilymphangiogenic therapy to promote transplant survival and to reduce cancer metastasis: what can  we learn from the eye? Semin Cell Dev Biol. 2015 Feb;38:117-30.

Cursiefen C, Viaud E, Bock F, Geudelin B, Ferry A, Kadlecová P, Lévy M, Al Mahmood S, Colin S, Thorin E, Majo F, Frueh B, Wilhelm F, Meyer-Ter-Vehn T, Geerling G, Böhringer D, Reinhard T, Meller D, Pleyer U, Bachmann B, Seitz B. Aganirsen antisense oligonucleotide eye drops inhibit keratitis-induced corneal neovascularization and reduce need for transplantation: the I-CAN study. Ophthalmology. 2014 Sep;121(9):1683-92.

Bock F, Rössner S, Onderka J, Lechmann M, Pallotta MT, Fallarino F, Boon L, Nicolette C, DeBenedette MA, Tcherepanova IY, Grohmann U, Steinkasserer A, Cursiefen C, Zinser E. Topical application of soluble CD83 induces IDO-mediated immune modulation, increases Foxp3+ T cells, and prolongs allogeneic corneal graft survival. J Immunol. 2013 Aug 15;191(4):1965-75.

Bock F, Maruyama K, Regenfuss B, Hos D, Steven P, Heindl LM, Cursiefen C. Novel anti(lymph)angiogenic treatment strategies for corneal and ocular surface diseases. Prog Retin Eye Res. 2013 May;34:89-124.

Cursiefen C, Maruyama K, Bock F, Saban D, Sadrai Z, Lawler J, Dana R, Masli S. Thrombospondin 1 inhibits inflammatory lymphangiogenesis by CD36 ligation on monocytes. J Exp Med. 2011 May 9;208(5):1083-92.

Dietrich T*, Bock F*, Yuen D, Hos D, Bachmann BO, Zahn G, Wiegand S, Chen L, Cursiefen C. Cutting edge: lymphatic vessels, not blood vessels, primarily mediate immune rejections after transplantation. J Immunol. 2010 Jan 15;184(2):535-9. *contributed equally

Cursiefen C, Chen L, Borges LP, Jackson D, Cao J, Radziejewski C, D’Amore PA,
Dana MR, Wiegand SJ, Streilein JW. VEGF-A stimulates lymphangiogenesis and
hemangiogenesis in inflammatory neovascularization via macrophage recruitment. J
Clin Invest. 2004 Apr;113(7):1040-50.